A 72-base pair AT-rich DNA sequence element functions as a bacterial gene silencer.

@article{Chen2001A7P,
  title={A 72-base pair AT-rich DNA sequence element functions as a bacterial gene silencer.},
  author={C C Chen and Ming Fang and Arundhati Majumder and Huantao Wu},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 12},
  pages={9478-85}
}
We have previously demonstrated that sequential activation of the bacterial ilvIH-leuO-leuABCD gene cluster involves a promoter-relay mechanism. In the current study, we show that the final activation of the leuABCD operon is through a transcriptional derepression mechanism. The leuABCD operon is transcriptionally repressed by the presence of a 318-base pair AT-rich upstream element. LeuO is required for derepressing the repressed leuABCD operon. Deletion analysis of the repressive effect of… CONTINUE READING

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