A 5' stem-loop and ribosome binding but not translation are important for the stability of Bacillus subtilis aprE leader mRNA.

@article{Hambraeus2002A5S,
  title={A 5' stem-loop and ribosome binding but not translation are important for the stability of Bacillus subtilis aprE leader mRNA.},
  author={Gustav Hambraeus and Kaisa Karhumaa and Blanka Rutberg},
  journal={Microbiology},
  year={2002},
  volume={148 Pt 6},
  pages={1795-803}
}
The Bacillus subtilis aprE leader is a determinant of extreme mRNA stability. The authors examined what properties of the aprE leader confer stability on an mRNA. The secondary structure of the aprE leader mRNA was analysed in vitro and in vivo, and mutations were introduced into different domains of an aprE leader-lacZ fusion. The half-lives of the corresponding transcripts were determined and beta-galactosidase activities were measured. Removal of a stem-loop structure at the 5' end or… CONTINUE READING

From This Paper

Figures and tables from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 39 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 53 references

The relationship between translational control and mRNA degradation for the Escherichia coli threonyl-tRNA synthetase gene

  • T. Nogueira, M. de Smit, M. Graffe, M. Springer
  • J Mol Biol
  • 2001
2 Excerpts

Similar Papers

Loading similar papers…