A 454 multiplex sequencing method for rapid and reliable genotyping of highly polymorphic genes in large-scale studies

@inproceedings{Galan2009A4M,
  title={A 454 multiplex sequencing method for rapid and reliable genotyping of highly polymorphic genes in large-scale studies},
  author={Maxime Galan and Emmanuel Guivier and Gilles Caraux and Nathalie Charbonnel and Jean-François Cosson},
  booktitle={BMC Genomics},
  year={2009}
}
High-throughput sequencing technologies offer new perspectives for biomedical, agronomical and evolutionary research. Promising progresses now concern the application of these technologies to large-scale studies of genetic variation. Such studies require the genotyping of high numbers of samples. This is theoretically possible using 454 pyrosequencing, which generates billions of base pairs of sequence data. However several challenges arise: first in the attribution of each read produced to its… CONTINUE READING
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