A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.

@article{Anand1990A3G,
  title={A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.},
  author={Rakesh L. Anand and John H. Riley and R. Butler and Jill Clayton Smith and Alex F. Markham},
  journal={Nucleic acids research},
  year={1990},
  volume={18 8},
  pages={1951-6}
}
The construction of a yeast artificial chromosome (YAC) primary gridded library of 35,000 clones from human lymphoblastoid (48,XXXX) cell line DNA is described. The average YAC size is approximately 350kb representing a greater than 3.5 times coverage of the genome. The library is stored at -70 degrees C as gridded clones on nylon filters impregnated with 20% glycerol and as glycerol suspensions of individual clones in microtitre plates providing a prolonged multi-user potential. To date we… CONTINUE READING