A 3'-coterminal nested set of independently transcribed mRNAs is generated during Berne virus replication.

@article{Snijder1990A3N,
  title={A 3'-coterminal nested set of independently transcribed mRNAs is generated during Berne virus replication.},
  author={Eric J. Snijder and Marian C. Horzinek and Willy J. M. Spaan},
  journal={Journal of virology},
  year={1990},
  volume={64 1},
  pages={331-8}
}
By using poly(A)-selected RNA from Berne virus (BEV)-infected embryonic mule skin cells as a template, cDNA was prepared and cloned in plasmid pUC9. Recombinants covering a contiguous sequence of about 10 kilobases were identified. Northern (RNA) blot hybridizations with various restriction fragments from these clones showed that the five BEV mRNAs formed a 3'-coterminal nested set. Sequence analysis revealed the presence of four complete open reading frames of 4743, 699, 426, and 480… CONTINUE READING

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