A 2D reversed-phase × ion-pair reversed-phase HPLC-MALDI TOF/TOF-MS approach for shotgun proteome analysis

@article{Lasaosa2009A2R,
  title={A 2D reversed-phase × ion-pair reversed-phase HPLC-MALDI TOF/TOF-MS approach for shotgun proteome analysis},
  author={Maria Lasaosa and Nathana{\"e}l Delmotte and Christian G. Huber and Katja Melchior and Elmar Heinzle and Andreas Tholey},
  journal={Analytical and Bioanalytical Chemistry},
  year={2009},
  volume={393},
  pages={1245-1256}
}
The separation of complex peptide mixtures in shotgun proteome analysis using a 2D separation scheme encompassing reversed-phase × ion-pair reversed-phase (IP-RP) liquid chromatography coupled online to electrospray ion trap mass spectrometry (MS) has been shown earlier to be superior in terms of separation efficiency and technical robustness compared to the classically used separation scheme encompassing strong cation exchange × IP-RP-chromatography in shotgun proteome analysis. In the present… CONTINUE READING

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