A 2.5 kb polypyrimidine tract in the PKD1 gene contains at least 23 H-DNA-forming sequences.

@article{Raay1996A2K,
  title={A 2.5 kb polypyrimidine tract in the PKD1 gene contains at least 23 H-DNA-forming sequences.},
  author={T J Van Raay and Timothy C. Burn and Timothy D. Connors and L R Petry and Gregory G Germino and Katherine Wood Klinger and Gregory M Landes},
  journal={Microbial & comparative genomics},
  year={1996},
  volume={1 4},
  pages={317-27}
}
A pyrimidine-rich element (PyRE), present in the 21st intron of the PKD1 gene, posed a significant obstacle in determining the primary structure of the gene. Only cycle sequencing of nested, single-stranded phage templates of the CT-rich strand enabled complete and accurate sequence data. Similar attempts on the GA-rich strand were unsuccessful. The… CONTINUE READING