A 1H nmr study of the interaction of aurothiomalate ("Myocrisin") with human red blood cells in vitro.

  title={A 1H nmr study of the interaction of aurothiomalate ("Myocrisin") with human red blood cells in vitro.},
  author={Gabriel Otiko and Muhammad Tahir Razi and Peter J. Sadler and A A Isab and Dallas L. Rabenstein},
  journal={Journal of inorganic biochemistry},
  volume={19 3},
The results of 1H spin-echo Fourier transform (SEFT) nuclear magnetic resonance (nmr) experiments suggest that some aurothiomalate binds intracellular glutathione (GSH) when added to suspensions of red cells in vitro. When added to red cell lysates, a specific binding of gold to cysteine of GSH is observed together with release of thiomalate. Gold binding to GSH can be reversed by addition of dimercaptopropanol sulfonate. Spectra are compared to those of an aurothiomalate-GSH model system. The… 
The 13C NMR study of the binding of gold(I) thiomalate with ergothionine in aqueous solution.
  • A. Isab
  • Chemistry, Medicine
    Journal of inorganic biochemistry
  • 1992
The interaction of gold(I) thiomalate (Autm) (Myocrysine) with ergothionine (ErSH) has been studied and it was found that ErSH forms a ternary complex of the type ErS-Au-tm at a 1:1 mole ratio; unlike other thiols it does not eject thiomicate (Htm) as a free ligand.
Progress in the characterization of gold drugs
NMR methods are used to examine in vitro the partition of auranofin between plasma and blood cells and the displacement of the thioglucose ligand and release of PEt3 from gold as OPEt3 are discussed.
Changes in glutathione in intact erythrocytes during incubation with penicillamine as detected by 1H spin-echo NMR spectroscopy.
1H spin-echo NMR spectroscopy was used to study changes in glutathione status in intact erythrocytes and it was observed that the methods of separation and suspension affect lactate metabolism in red cells.
Reactions of model proteins with aurothiomalate, a clinically established gold(I) drug: The comparison with auranofin.
Key characteristic and rather constant features emerged in the protein metalation patterns induced by AuTm that are markedly distinct from those caused by auranofin; a non-covalent interaction mode is invoked for AuTtm binding to the mentioned proteins.
Competition between gold(I) thiomalate ‘myocrisin’ and five-member and six-member heterocyclic ligands
Abstract The interactions of gold(I) thiomalate (‘Myocrisin’) [Au(tm)] with imidazolidine-2-thione [Imt] (a five-member heterocyclic ligand) and 1,3- diazinane-2-thione [Diaz] (a six-member
NMR Spectroscopy of Biofluids
Publisher Summary This chapter reviews that the analysis of biofluids provides a unique window into the biochemical status of a living organism since the composition of a given biofluid modulates
Reaction of cis- and trans-[PtCl2(NH3)2] with reduced glutathione inside human red blood cells, studied by 1H and 15N-[1H] DEPT NMR.
There was a gradual decrease in the intensity of the GSH 1H spin-echo NMR resonances, but no new peaks were resolved, which was interpreted as formation of high-molecular weight Pt:GSH and mixed GS-Pt-S(hemoglobin) polymers.
A reaction between captopril (a high blood pressure drug) and gold(I)-thiomalate.
  • A. Isab
  • Chemistry, Medicine
    Journal of inorganic biochemistry
  • 1987
A reaction of gold(I) thiomalates [Au(tm)], "Myocrisin" (an antiarthritic drug), with captopril (a high blood pressure drug) was carried out in aqueous solution at pH 7.20 using 13C NMR spectroscopy.
13C n.m.r. studies of the interaction of HgII with methionine and selenamethionine in the acidic aqueous solution
SummaryThe interactions of L-methionine (Met) and DL-selenamethionine (SeMet) with Hg2+ were studied using13C n.m.r. spectroscopy in aqueous acid. Hg2+ binds to the selenoether group of SeMet and the
Abstract The interaction of thiourea (TU) and selenourea (SeU) with aurothiomalate (Autm) has been studied by 13C NMR spectroscopy. At a 1:1 ratio of TU:Autm, TU binds to Autm forming a ternary


The complexation of zinc in intact human erythrocytes studied by 1H spin‐echo NMR
Results are reported which demonstrate for the first time that the binding of metal ions by mole- cules in an intact cellular system can be studied directly at the molecular level by ‘H NMR.
Endogenous phospholipase and choline release in human erythrocytes: a study using 1H-NMR spectroscopy.
1H spin-echo NMR spectroscopy has been used to show for the first time the presence of a phospholipase D activity in human erythrocytes, and it is demonstrated that it is activated by a component of Krebs bicarbonate medium; most probably calcium ions.
Human erythrocyte metabolism studies by 1H spin echo NMR
NMR has been added to this list of techniques by the use of both 31P and 13C in studies of whole red cells by allowing a non-invasive inspection of even the most delicately balanced system.
Transport of gold in the body.
Gold compounds continue to be used in rheumatoid arthritis and there is good evidence to indicate that they make an effective contribution to therapy, but there is little information on how gold is carried by the blood.
Metabolism, Toxicity and Manner of Action of Gold Compounds used in the Treatment of Arthritis
The rate of excretion of gold is parallel to the absorption rate for each compound, but is incomplete eighty-five days after the last injection even in the case of sodium succinimido aurate which is the most rapidly excreted substance.