A 16S rRNA-based PCR assay for detection and identification of granulocytic Ehrlichia species in dogs, horses, and cattle

@article{Engvall1996A1R,
  title={A 16S rRNA-based PCR assay for detection and identification of granulocytic Ehrlichia species in dogs, horses, and cattle},
  author={E. Engvall and B. Pettersson and M. Persson and K. Artursson and K. Johansson},
  journal={Journal of Clinical Microbiology},
  year={1996},
  volume={34},
  pages={2170 - 2174}
}
A PCR-based assay was developed for detecting DNA of granulocytic ehrlichiae in blood samples from dogs, horses, and cattle, Primers were designed from 16S rRNA sequence information to specifically amplify DNA from a newly identified Swedish Ehrlichia species. The 16S rRNA nucleotide sequence of this Swedish species differs in only two and three positions from the sequences of Ehrlichia phagocytophila and Ehrlichia equi, respectively, which were also amplified by this PCR system. For evaluation… Expand
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  • Biology, Medicine
  • Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria
  • 2010
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Seven Swedish isolates of Ehrlichia species from the blood of four dogs and three horses with clinical granulocytic ehrlichiosis, were identified by direct solid phase DNA sequencing of polymeraseExpand
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Six patients from northern Minnesota and Wisconsin with a febrile illness accompanied by granulocytic cytoplasmic morulae suggestive of ehrlichial infection were identified and were shown to be infected by an Ehrlichia species never previously reported to infect humans. Expand
Comparison of PCR with other tests for early diagnosis of canine ehrlichiosis
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The sensitivity of PCR is comparable to or slightly less than that of other established methods; however, the convenience, quickness, and direct nature of detecting E. canis DNA is expected to make PCR more useful for clinical diagnosis. Expand
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The sequence comparisons indicate that the human ehrlichiosis agent is a new species most closely related to E. canis and more distantly related to other Ehrlichia spp, and it is proposed that this species be named EHRlichia chaffeensis sp. Expand
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The sequences of the 16S rRNA genes of several isolates obtained from sick horses diagnosed as having Potomac horse fever may represent two new distinct Ehrlichia species. Expand
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TLDR
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TLDR
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TLDR
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TLDR
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