A 13 kb resolution cosmid map of the 14 Mb fission yeast genome by nonrandom sequence-tagged site mapping.

@article{Mizukami1993A1K,
  title={A 13 kb resolution cosmid map of the 14 Mb fission yeast genome by nonrandom sequence-tagged site mapping.},
  author={Toru Mizukami and Won Il Chang and Igor Garkavtsev and Nachum Kaplan and D Lombardi and Tomohiro Matsumoto and Osami Niwa and Asako Kounosu and Mitsuhiro Yanagida and Thomas G. Marr},
  journal={Cell},
  year={1993},
  volume={73 1},
  pages={121-32}
}
We present the application of a nonrandom sequence-tagged site (STS) content detection method in mapping an entire genome, that of fission yeast. The novelty of our strategy is in the use of STS probes made from both ends of cosmid clones, selected on the basis of "sample without replacement" (only library clones that show no previous positive hybridization… CONTINUE READING