A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans

@inproceedings{Wang2016AC,
  title={A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans},
  author={Yu Wang and Dong-sheng Wei and Xiangyang Zhu and Jiao Pan and Ping Zhang and Liang Huo and Xudong Zhu},
  booktitle={Scientific reports},
  year={2016}
}
Loss-of-function mutagenesis is an important tool used to characterize gene functions, and the CRISPR-Cas9 system is a powerful method for performing targeted mutagenesis in organisms that present low recombination frequencies, such as the serotype D strains of Cryptococcus neoformans. However, when the CRISPR-Cas9 system persists in the host cells, off-target effects and Cas9 cytotoxicity may occur, which might block subsequent genetic manipulation. Here, we report a method of spontaneously… CONTINUE READING
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