A (1-->3,1-->4)-beta-glucan-specific monoclonal antibody and its use in the quantitation and immunocytochemical location of (1-->3,1-->4)-beta-glucans.

@article{Meikle1994AM,
  title={A (1-->3,1-->4)-beta-glucan-specific monoclonal antibody and its use in the quantitation and immunocytochemical location of (1-->3,1-->4)-beta-glucans.},
  author={Peter J. Meikle and Nicholas J. Hoogenraad and Ingrid Bonig and Adrienne Clarke and Bruce A. Stone},
  journal={The Plant journal : for cell and molecular biology},
  year={1994},
  volume={5 1},
  pages={1-9}
}
Monoclonal antibodies were raised against a (1-->3,1-->4)-beta-glucan-bovine serum albumin (BSA) conjugate. One antibody (BG1) selected for further characterization, was specific for (1-->3,1-->4)-beta-glucan, displaying no binding activity against a (1-->3)-beta-glucan-BSA conjugate and minimal binding against a cellopentaose-BSA conjugate. A range of oligosaccharides was prepared by enzymatic digestion of (1-->3,1-->4)-beta-glucan, purified by size exclusion chromatography and characterized… CONTINUE READING

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