A "tagless" strategy for identification of stable protein complexes genome-wide by multidimensional orthogonal chromatographic separation and iTRAQ reagent tracking.

@article{Dong2008AS,
  title={A "tagless" strategy for identification of stable protein complexes genome-wide by multidimensional orthogonal chromatographic separation and iTRAQ reagent tracking.},
  author={Ming Dong and Lee Lisheng Yang and Katherine Nguyen Williams and Susan J. Fisher and Steven C Hall and Mark D. Biggin and Jian Jin and Halina Ewa Witkowska},
  journal={Journal of proteome research},
  year={2008},
  volume={7 5},
  pages={1836-49}
}
Tandem affinity purification is the principal method for purifying and identifying stable protein complexes system-wide in whole cells. Although highly effective, this approach is laborious and impractical in organisms where genetic manipulation is not possible. Here, we propose a novel "tagless" strategy that combines multidimensional separation of endogenous complexes with mass spectrometric monitoring of their composition. In this procedure, putative protein complexes are identified based on… CONTINUE READING

From This Paper

Topics from this paper.
13 Citations
35 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 13 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 35 references

Methodological Refinements in iTRAQ  ReagentBased “ Tagless ” Strategy of Identification and Purification of Soluble Protein Complexes in Bacteria

  • M. Dong, H. Liu, +9 authors H. E. Witkowska
  • Presented at the 8 th International Symposium on…
  • 2007

Multi - dimensional orthogonal separation and iTRAQTM reagent tracking : a genome - wide “ tagless ” strategy for isolation and detection of soluble bacterial protein complexes

  • M. Dong, M. D. Biggin, +6 authors H. E. Witkowska
  • Presented at the 54 th ASMS Conference on Mass…
  • 2006

Similar Papers

Loading similar papers…