Corpus ID: 26508490

A "molten globule" of Torpedo acetylcholinesterase undergoes thiol-disulfide exchange.

  title={A "molten globule" of Torpedo acetylcholinesterase undergoes thiol-disulfide exchange.},
  author={Jerry Eichler and David I. Kreimer and L E Varon and Israel Silman and L. Weiner},
  journal={The Journal of biological chemistry},
  volume={269 48},
  • Jerry Eichler, David I. Kreimer, +2 authors L. Weiner
  • Published in
    The Journal of biological…
  • Chemistry, Medicine
  • Torpedo acetylcholinesterase is a disulfide-linked homodimer containing three intramolecular disulfide bonds, as well as a single free thiol on Cys-231. We report that in a "molten globule" state, produced by 1.5 M guanidine hydrochloride, this enzyme undergoes rapid intramolecular thiol-disulfide exchange, in the absence of reducing agents, resulting in the production of novel species. Most strikingly, this results in appearance of enzyme monomers. Chemical modification of the free thiol group… CONTINUE READING

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