A/T mutagenesis in hypermutated immunoglobulin genes strongly depends on PCNAK164 modification

@article{Langerak2007ATMI,
  title={A/T mutagenesis in hypermutated immunoglobulin genes strongly depends on PCNAK164 modification},
  author={Petra Langerak and Anders O. H. Nygren and Peter H. L. Krijger and Paul C.M. van den Berk and Heinz Jacobs},
  journal={The Journal of Experimental Medicine},
  year={2007},
  volume={204},
  pages={1989 - 1998}
}
B cells use translesion DNA synthesis (TLS) to introduce somatic mutations around genetic lesions caused by activation-induced cytidine deaminase. Monoubiquitination at lysine(164) of proliferating cell nuclear antigen (PCNA(K164)) stimulates TLS. To determine the role of PCNA(K164) modifications in somatic hypermutation, PCNA(K164R) knock-in mice were generated. PCNA(K164R/K164R) mutants are born at a sub-Mendelian frequency. Although PCNA(K164R/K164R) B cells proliferate and class switch… CONTINUE READING