99mTc-labeled single-domain antibody EG2 in targeting epidermal growth factor receptor: an in-vitro and mouse model in-vivo study

@article{Li201599mTclabeledSA,
  title={99mTc-labeled single-domain antibody EG2 in targeting epidermal growth factor receptor: an in-vitro and mouse model in-vivo study},
  author={Chongjiao Li and Bing Wen and Lifei Wang and Hongyan Feng and Xiaotian Xia and Zhi-qiang Ding and Bin Gao and Yongxue Zhang and Xiaoli Lan},
  journal={Nuclear Medicine Communications},
  year={2015},
  volume={36},
  pages={452–460}
}
ObjectiveThe aim of this study was to explore the targeting ability and metabolic characteristics of the technetium-99m-labeled single-domain antibody (99mTc-sdAb) EG2 targeting epidermal growth factor receptor (EGFR) through an in-vitro and in-vivo study. Materials and methodsThe sdAb EG2 was radiolabeled with 99mTc using a tricarbonyl kit. The EGFR expression level of A431 and OCM-1 cells was confirmed using immunofluorescence staining. Cell binding, blocking, uptake, and efflux studies were… Expand
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