950 a Novel Fluorescence Technique for Determining Bilirubin Binding Constants (lipids and Albumin) and Serum Bilirubin (free and Total)


The interaction of bilirubin with various lipids was studied utilizing a novel approach - a static fluorescence quenching method. A quenching equation was developed to determine bilirubin binding constants. The method was tested first by a dansyl bovine albumin system which yielded an association constant of 3.5±1.6 × 107M−1 and then applied to fluorescence… (More)
DOI: 10.1203/00006450-197804001-00956


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