8-Mercaptoflavins as active site probes of flavoenzymes.

  title={8-Mercaptoflavins as active site probes of flavoenzymes.},
  author={Vincent Massey and Sandro Ghisla and Elizabeth G. Moore},
  journal={The Journal of biological chemistry},
  volume={254 19},
Representative examples of the various classes of flavoproteins have been converted to their apoprotein forms and the native flavin replaced by 8-mercapto-FMN or 8-mercapto-FAD. The spectral and catalytic properties of the modified enzymes are characteristically different from one group to another; the results suggest that flavin interactions at positions N(1) or N(5) of the flavin chromophore have profound influences on the properties of the flavoprotein. 1. The 8-thiolate anion form of 8… CONTINUE READING


Publications referenced by this paper.


  • Biochem
  • 1977


  • S Quay, V Massey
  • Biochemistry
  • 1977

J. Mol. Biol

  • J. Mol. Biol
  • 1977

Progress in the Chemistry of Organic Natural Products

  • G Blankenhorn, K Yagi, T Yamano
  • Progress in the Chemistry of Organic Natural…
  • 1976

J. Biochem

  • J. Biochem
  • 1975

J. Biol

  • R G Matthews, V Massey, C C Sweeley
  • J. Biol
  • 1975

Proceedings Tenth FEBS Meeting

  • S Ghisla, V Massey
  • Proceedings Tenth FEBS Meeting
  • 1975

Biochemistry, in press Becvar

  • S T Olson, V Massey
  • J. E. Eur. J. Biochem
  • 1973

Biochim. Biophys. Actu

  • B Curti, S Ronchi, U Branzoli, G Ferri, Williams
  • Biochim. Biophys. Actu
  • 1973

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