8-Hydroxydeoxyguanosine in DNA from TPA-stimulated human granulocytes.

@article{Birnboim19948HydroxydeoxyguanosineID,
  title={8-Hydroxydeoxyguanosine in DNA from TPA-stimulated human granulocytes.},
  author={H Chaim Birnboim and L Maidt and T Raynor and Robert A. Floyd},
  journal={Free radical research},
  year={1994},
  volume={20 2},
  pages={113-7}
}
8-Hydroxydeoxyguanosine (8-OHdG) is now widely used as a sensitive marker of oxidative damage to DNA. When human granulocytes are stimulated with TPA, they release a large quantity of reactive oxygen species (superoxide, hydrogen peroxide) which might be expected to generate hydroxyl radicals (OH.) which in turn could produce 8-OHdG in the DNA. There had been considerable debate as to whether OH. is detectable in stimulated granulocytes; most workers now agree that none can be detected, unless… CONTINUE READING