76Br-labeled monoclonal anti-CEA antibodies for radioimmuno positron emission tomography.

Abstract

For the application of anti-tumor monoclonal antibodies (MAbs) in positron emission tomography (PET), labeling radionuclides with half-lives allowing a suitable time frame for imaging are required. The anti-CEA MAb 38S1 was labeled with the positron emitting nuclide 76Br (t1/2 16 h) using bromoperoxidase (BPO), and subsequently affinity purified. A procedure was devised to allow reproducible production of MAb-preparations of high immunoreactivity and with acceptable bromination yield. The biological activity of 76Br-38S1 was retained and comparable to that of chloramine-T labeled 125I-38S1, as tested in vitro.

Cite this paper

@article{Lvqvist199576BrlabeledMA, title={76Br-labeled monoclonal anti-CEA antibodies for radioimmuno positron emission tomography.}, author={A L{\"{o}vqvist and Anders Sundin and H{\aa}kan Ahlstr{\"{o}m and J{\"{o}rgen Carlsson and Hans Lundqvist}, journal={Nuclear medicine and biology}, year={1995}, volume={22 1}, pages={125-31} }