7-Ethoxycoumarin deethylase activity as a convenient measure of liver drug metabolizing enzymes: regulation in cultured rat hepatocytes.

@article{Edwards19847EthoxycoumarinDA,
  title={7-Ethoxycoumarin deethylase activity as a convenient measure of liver drug metabolizing enzymes: regulation in cultured rat hepatocytes.},
  author={Anthony M. Edwards and M. L. Glistak and C. M. Lucas and Paul A. Wilson},
  journal={Biochemical pharmacology},
  year={1984},
  volume={33 9},
  pages={1537-46}
}
Assays of 7-ethoxycoumarin O-deethylase (ECD) activity in intact cells were used as a sensitive and convenient measure of the drug-metabolizing activity of rat hepatocytes maintained for up to 4 days in primary culture. A combination of nicotinamide or other pyridines with dexamethasone was shown to maintain ECD at or above the activity of untreated livers in vivo and to potentiate induction by xenobiotics. Inductions in vivo and in culture were quantitatively similar but differed qualitatively… CONTINUE READING