6R-[3H]tetrahydrobiopterin binding activities in rat brain.

  title={6R-[3H]tetrahydrobiopterin binding activities in rat brain.},
  author={Y. Watanabe and Hiroshi Morii and Yasuo Nemoto and Bernd Mayer and Ernst R. Werner and Soichi Miwa and Y. Watanabe},
  journal={Advances in experimental medicine and biology},
6R-L-Erythro-5,6,7,8-Tetrahydrobiopterin (R-THBP) is a common cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases, the latter two of which are the rate-limiting enzymes of catecholamines and serotonin biosyntheses, respectively. In an attempt to answer the puzzle from the clinical evaluation that R-THBP and L-DOPA or 5-HTP possess the additive effect on the treatment of atypical phenylketonuria, Parkinsonian disease, and infantile autism, we recently found a novel role of R-THBP… Expand
Maturation alters cerebral NOS kinetics in the spontaneously hypertensive rat.
The hypotheses that maturation increases neuronal nitric oxide synthase (nNOS) activity and that this increase involves changes in cofactor availability and/or nNOS kinetics are supported, which support the general hypothesis that the increased vulnerability to ischemic stroke associated with maturation is due in part to corresponding increases in the capacity for Nitric oxide synthesis. Expand
Maturation alters cerebral NOS kinetics in the spontaneously hypertensive rat.
  • W. Pearce, B. Tone, S. Ashwal
  • Chemistry, Medicine
  • American journal of physiology. Regulatory, integrative and comparative physiology
  • 1997
The results indicate that endogenous nNOS co Factor levels are not saturating in either pups or adults, changes in cofactor levels differentially affect NOS kinetics in pups and adults, and age-related differences in NOS activity result from fundamental differences inNOS Kinetics. Expand
Nitric oxide and biopterin in depression and stress
The evidence linking tetrahydrobiopterin and nitric oxide, an apparent neuroendocrine modulator of the HPA axis, to the immune system and to neuronal control within affective disorder and stress is reviewed. Expand


Molecular Mechanisms of Tetrahydrobiopterin Action on Neurotransmitter Release
6R-L-erythro-5,6,7,8-tetrahydrobiopterin (R-THBP) is a common cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases, the latter two of which are the rate-limiting enzymes ofExpand
6R-tetrahydrobiopterin perfusion enhances dopamine, serotonin, and glutamate outputs in dialysate from rat striatum and frontal cortex
Results suggest that R-THBP may play a role in the mechanisms of release of dopamine, serotonin, and glutamate in urethane-anesthetized rats and the functioning of the catecholaminergic system probably mediates the increase in glutamate output due to R- THBP perfusion. Expand
Monoamine-mediated enhancement of acetylcholine release in rat hippocampus by 6R-l-erythro-5, 6, 7, 8-tetrahydrobiopterin
The results show that 6R-BH4 stimulates exocytotic ACh release in the hippocampus through action on a local monoaminergic system in the hippocampal region of rats pretreated with reserpine to deplete monoamines. Expand
Enhancement of Dopamine Release In Vivo from the Rat Striatum by Dialytic Perfusion of 6R‐l‐erythro‐5,6,7,8‐Tetrahydrobiopterin
The results suggest that 6R‐BH4 has a dopamine‐releasing action, which is not dependent on biosynthesis of dopamine. Expand
Solubilization and Characterization of Prostaglandin E2 Binding Protein from Porcine Cerebral Cortex
Results suggest that a specific protein solubilized may be responsible for the binding site of prostaglandin E2. Expand
Brain nitric oxide synthase is a biopterin‐ and flavin‐containing multi‐functional oxido‐reductase
It is found that purified NO synthase contains enzyme‐bound H4 biopterin, explaining the enzymatic activity observed in the absence of added cofactor, and these results indicate that No synthase essentially depends on H4Biopterin as a cofactor which is recycled during enzymatically NO formation. Expand
Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme.
  • D. Bredt, S. Snyder
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1990
It is shown that nitric oxide synthetase activity requires calmodulin, and the native enzyme appears to be a monomer. Expand