6-Thioguanine in DNA as CD-spectroscopic probe to study local structural changes upon protein binding.

Abstract

A combination of experimental and theoretical circular dichroism (CD) spectroscopy was used to study local deformations of DNA caused by binding of the base flipping DNA methyltransferase M.TaqI. To selectively study the structural changes within the DNA, we replaced single guanine residues at six different positions in duplex DNA with 6-thioguanine (s(6)G… (More)
DOI: 10.1002/chir.20540

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