6-N-1 Trobenzotriazol-1-Yloxytris(Dimethylamino)Phosphonium Hexafluorophosphate (NBOP): A New Coupling Reagent in Oligonucleotide Synthesis

@article{Hashmi19946N1TH,
  title={6-N-1 Trobenzotriazol-1-Yloxytris(Dimethylamino)Phosphonium Hexafluorophosphate (NBOP): A New Coupling Reagent in Oligonucleotide Synthesis},
  author={S. A. Nadeem Hashmi and M. Adiraj and Anil Kumar and Seturam B Katti},
  journal={Nucleosides, Nucleotides \& Nucleic Acids},
  year={1994},
  volume={13},
  pages={1059-1067}
}
Abstract 6-Nitrobenzotriazol-1-yloxytris(dimethylamino) phosphonium hexafluorophosphate (NBOP, 2) has been demonstrated as an efficient coupling reagent in oligonucleotide synthesis. The reagent is stable and gives high coupling yields without any side products. 
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6 Citations

6 Citations

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6-Nitrobenzotriazol-1-yloxytris(dimethylamino)phosphonium Hexafluorophosphate (NBOP): A New Coupling Reagent in Oligonucleotide Synthesis.

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TLDR
1-Mesitylenesulphonyl-3-nitro-1,2,4-triazole is used as the activating agent in the second phosphorylation step in which 5'-protected mono- and di-nucleotides are condensed with nucleoside building blocks containing unprotected 3'-hydroxy functions.
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