5-Methyltetrahydrofolate Transport by Hepatoma Cells and Methotrexate-resistant Sublines in Culture 1

@inproceedings{Galivan20065MethyltetrahydrofolateTB,
  title={5-Methyltetrahydrofolate Transport by Hepatoma Cells and Methotrexate-resistant Sublines in Culture 1},
  author={John Galivan},
  year={2006}
}
The properties of the folate transport system in H35 hepatoma cells have been studied by measuring the transport of (+)-5-methyltetrahydrofolate. Using initial rates of uptake, it has been demonstrated that the uptake is saturable, carrier mediated, and shared by methotrexate. The accumulation of ( + )-5-methyltetrahydrofoÃ-ate is concentrative, demonstrating the presence of an active transport process. A previous study suggested that methotrexate-resistant sublines (H35R) acquired methotrexate… CONTINUE READING