5-Methoxysalicylic acid and spermine: A new matrix for the matrix-assisted laser desorption/ionization mass spectrometry analysis of oligonucleotides

@article{Distler20015MethoxysalicylicAA,
  title={5-Methoxysalicylic acid and spermine: A new matrix for the matrix-assisted laser desorption/ionization mass spectrometry analysis of oligonucleotides},
  author={Anne M. Distler and John. Allison},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2001},
  volume={12},
  pages={456-462}
}
  • A. Distler, J. Allison
  • Published 2001
  • Chemistry, Medicine
  • Journal of the American Society for Mass Spectrometry
Abstract5-Methoxysalicylic acid (MSA) is demonstrated to be a useful matrix for matrix-assisted laser desorption/ionization time-of-flight (TOF) mass spectrometry of oligonucleotides, when desorption/ionization without fragmentation is desired. When MSA is combined with the additive spermine, the need for desalting is reduced. The MSA/spermine matrix yields linear TOF mass spectra with improved resolution, less fragmentation, and less intense alkali ion adduct peaks than those spectra obtained… Expand
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References

SHOWING 1-10 OF 73 REFERENCES
Enhanced detection of oligonucleotides in UV MALDI MS using the tetraamine spermine as a matrix additive.
TLDR
It is found that tetraamine spermine as a matrix additive can successfully eliminate cation adduction and lower the detection limits for DNA in the MALDI experiment, without having to resort to desalting steps. Expand
Matrix-assisted laser desorption/ionization mass spectrometry methods for oligodeoxynucleotides: Improvements in matrix, detection limits, quantification, and sequencing
  • L. Zhang, M. Gross
  • Chemistry, Medicine
  • Journal of the American Society for Mass Spectrometry
  • 2000
A comatrix of anthranilic acid and nicotinic acid is optimum for the matrix-assisted laser desorption/ionization time of flight determination of oligodeoxynucleotides that are comprised of up to 21Expand
Matrix-assisted laser desorption time-of-flight mass spectrometry of oligonucleotides using 3-hydroxypicolinic acid as an ultraviolet-sensitive matrix.
TLDR
Results were obtained with a new matrix material, 3-hydroxypicolinic acid (3-Hydroxypyridine-2-carboxylic acid), which showed significant improvement over many previously reported matrices studied in terms of mass range available, signal-to-noise ratio, and the ability to analyze mixed-base oligomers. Expand
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a powerful tool for the mass and sequence analysis of natural and modified oligonucleotides.
TLDR
This methodology can be used in combination with time dependent degradation of oligonucleotides by exonucleases as powerful tool to determine sequence compositions. Expand
Analysis of oligodeoxynucleotides by negative-ion matrix-assisted laser desorption mass spectrometry
  • G. Currie, J. Yates
  • Chemistry, Medicine
  • Journal of the American Society for Mass Spectrometry
  • 1993
TLDR
The results of these studies have shown that both sensitivity and desorption conditions need to be further improved before complex mixtures of large pieces of DNA can be effectively analyzed. Expand
Matrix-assisted laser desorption/ionization mass spectrometry of synthetic oligodeoxyribonucleotides
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has been used to mass analyze a variety of synthetic oligodeoxyribonucleotides ranging in size from 6 to 30 nucleotides.Expand
New matrices and accelerating voltage effects in matrix‐assisted laser desorption/ionization of synthetic polymers
Most matrices used in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) have been selected for their sound performance in the detection of proteins. We evaluated a series ofExpand
Ultraviolet/matrix-assisted laser desorption/ionization mass spectrometric characterization of 2,5-dihydroxybenzoic acid-induced reductive hydrogenation of oligonucleotides on cytosine residues.
TLDR
It is proposed that cytosine is the site of reduction of DNA oligonucleotides, and the relative intensities of ion signals corresponding to the reduced base can be altered by using different matrix additives or a different substrate (copper). Expand
Applications of delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry to oligonucleotide analysis.
TLDR
A delayed ion extraction technique is shown to dramatically improve mass resolution and the overall quality of matrix-assisted laser desorption ionization (MALDI) mass spectra of oligonucleotides, and fast fragmentation taking place between the ionizing pulse and the extraction pulse is demonstrated to be a sequencing tool for small oligon nucleotides. Expand
Matrix-assisted ultraviolet laser desorption of non-volatile compounds
Abstract The use of a matrix for pulsed ultraviolet laser desorption mass spectrometry is shown to extend its applicability into the range of larger, thermally labile biomolecules. The matrixExpand
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