5-Fluorouracil incorporation in DNA of human breast carcinoma cells.

  title={5-Fluorouracil incorporation in DNA of human breast carcinoma cells.},
  author={Pierre P. Major and E. Michael Egan and David J Herrick and Donald Kufe},
  journal={Cancer research},
  volume={42 8},
We have demonstrated previously the presence of 5-fluorouracil (FUra) residues in L1210 DNA. These findings have been extended to the MCF-7 human breast carcinoma cell line. Cesium sulfate gradient centrifugation has been used to separate the MCF-7 RNA and DNA fractions. Alkali and RNase digests have also been used to remove any possible RNA contaminating the DNA fraction. The purified DNA has been analyzed by high-pressure liquid chromatography following digestion to nucleotides and… CONTINUE READING