5-Aminolevulinic acid induces single-strand breaks in plasmid pBR322 DNA in the presence of Fe2+ ions.

@article{Onuki19945AminolevulinicAI,
  title={5-Aminolevulinic acid induces single-strand breaks in plasmid pBR322 DNA in the presence of Fe2+ ions.},
  author={J Onuki and Marisa H G Medeiros and Etelvino Jos{\'e} Henriques Bechara and Paolo di Mascio},
  journal={Biochimica et biophysica acta},
  year={1994},
  volume={1225 3},
  pages={259-63}
}
5-Aminolevulinic acid (ALA), a heme precursor accumulated in chemical and inborn porphyrias, has been demonstrated to produce reactive oxygen species upon metal-catalyzed aerobic oxidation and to cause oxidative damage to proteins, liposomes and subcellular structures. Exposure of plasmid pBR322 DNA to ALA (0.01-3 mM) in the presence of 10 microM Fe2+ ions causes DNA single-strand breaks (ssb), revealed by agarose gel electrophoresis as an increase in the proportion of the open circular form… CONTINUE READING