5′ to 3′ Single Strand DNA Exonuclease Activity in a Preparation of Human Ku Protein

@article{Morozov19995T3,
  title={5′ to 3′ Single Strand DNA Exonuclease Activity in a Preparation of Human Ku Protein},
  author={V. E. Morozov and B. G. Fuller},
  journal={IUBMB Life},
  year={1999},
  volume={48}
}
  • V. E. Morozov, B. G. Fuller
  • Published 1999
  • Chemistry, Medicine
  • IUBMB Life
  • We describe a novel 5′ to 3′ single‐strand exonuclease activity exhibited by a Ku preparation purified from a human cell line. The enzyme removes 5′ single‐strand extensions from duplex DNA molecules. The exonuclease and helicase activities respond reciprocally to changes in ATP concentrations: Nuclease activity is inhibited at the ATP concentrations that are optimal for the helicase. The exonuclease activity does not require divalent cations. The potential implications of the exonuclease… CONTINUE READING

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