5′ end of hmg CoA reductase gene contains sequences responsible for cholesterol-mediated inhibition of transcription

@article{Osborne19855EO,
  title={5′ end of hmg CoA reductase gene contains sequences responsible for cholesterol-mediated inhibition of transcription},
  author={Timothy F. Osborne and Joseph L. Goldstein and Michael S. Brown},
  journal={Cell},
  year={1985},
  volume={42},
  pages={203-212}
}

Figures and Tables from this paper

Chimeric 3-hydroxy-3-methylglutaryl coenzyme A reductase-dihydrofolate reductase genes display bidirectional expression and unidirectional regulation in stably transfected cells.
TLDR
Stable transfection frequencies of these chimeric templates into a DHFR-deficient Chinese hamster cell line indicate that the HMG CoA reductase promoter fragment confers DHFR transformation irrespective of its orientation relative to a downstream murine DHFR cDNA.
Chimeric 3-hydroxy-3-methylglutaryl coenzyme A reductase-dihydrofolate reductase genes display bidirectional expression and unidirectional regulation in stably transfected cells
TLDR
Stable transfection frequencies of these chimeric templates into a DHFR-deficient Chinese hamster cell line indicate that the HMG CoA reductase promoter fragment confers DHFR transformation irrespective of its orientation relative to a downstream murine DHFR cDNA.
Conservation of promoter sequence but not complex intron splicing pattern in human and hamster genes for 3-hydroxy-3-methylglutaryl coenzyme A reductase
TLDR
The human HMG-CoA reductase gene resembles the Chinese hamster gene in having multiple sites of transcription initiation that are subject to suppression by cholesterol, indicating the interspecies conservation of the regulatory elements.
Nucleotide sequence and nuclease hypersensitivity of the Chinese hamster dihydrofolate reductase gene promoter region.
TLDR
Two clusters of short, G/C-rich elements conforming to the consensus binding sequence for the transcription factor Spl are located in the upstream region in all three genes, suggesting that the Spl protein may be involved in maintaining chromatin structure in this region.
Identification of a zinc finger protein that binds to the sterol regulatory element.
TLDR
A clone for a DNA binding protein was isolated that binds to the conserved SRE octanucleotide in both a sequence-specific and a single-strand--specific manner and is consistent with a role in sterol-mediated control of transcription.
Functional Analysis of the Hepatic HMG-CoA Reductase Promoter by In Vivo Electroporation
TLDR
It is found that HMGR promoter constructs were sterol responsive in live animals, adding in vivo relevance to previous findings in cultured cells and concluding that in vivo electroporation is a convenient and powerful technique for the analysis of promoter elements in the livers of live animals.
c-Ha-ras gene bidirectional promoter expressed in vitro: location and regulation
TLDR
Using S1 and primer extension analysis of c-Ha-ras RNA from EJ cells, 18 initiation sites within an upstream exon (exon -1) whose 3' end (the donor splice site [D]) is located 1,105 base pairs upstream of the ATG codon are identified.
The promoter of the rat 3-hydroxy-3-methylglutaryl coenzyme A reductase gene contains a tissue-specific estrogen-responsive region.
TLDR
Red-ERE is able to mediate hormonal regulation of the HMG CoA reductase gene in tissues that respond to estrogens with enhanced cell proliferation, while it is not operative in liver cells, postulated to partially explain the protective effect of estrogens against heart disease.
Regulation of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Gene Expression in FRTL-5 Cells
TLDR
The data presented provide the molecular basis for a novel regulatory mechanism for HMG-CoA reductase gene expression in rat thyroid cells, which involves the direct effect of cAMP.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 43 REFERENCES
HMG CoA reductase: A negatively regulated gene with unusual promoter and 5′ untranslated regions
Adenosine deaminase: characterization and expression of a gene with a remarkable promoter.
TLDR
Cosmid clones containing the gene for human adenosine deaminase (ADA) were isolated and it was shown in a functional assay that a stretch of 135 bp immediately preceding the cap site has promoter activity.
Sterols accelerate degradation of hamster 3-hydroxy-3-methylglutaryl coenzyme A reductase encoded by a constitutively expressed cDNA.
TLDR
It is demonstrated that sterols accelerate the degradation of reductase protein independently of any inhibitory effect on the synthesis of the protein, and this decline was caused by a twofold increase in the rate of degradation of preformed enzyme molecules.
Amplification of the gene for 3-hydroxy-3-methylglutaryl coenzyme A reductase, but not for the 53-kDa protein, in UT-1 cells.
TLDR
It is concluded that the gene for 3-hydroxy-3-methylglutaryl coenzyme A reductase, but not for the 53-kDa protein, has been stably amplified in UT-1 cells.
Multivalent feedback regulation of HMG CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth.
The availability of compactin (ML-236B), a potent competitive inhibitor of 3-hydroxy-3-methylglutaryl Coenzyme A reductase, has permitted the demonstration of a hitherto unsuspected aspect of
...
1
2
3
4
5
...