5′ end of hmg CoA reductase gene contains sequences responsible for cholesterol-mediated inhibition of transcription

@article{Osborne19855EO,
  title={5′ end of hmg CoA reductase gene contains sequences responsible for cholesterol-mediated inhibition of transcription},
  author={Timothy F. Osborne and Joseph L. Goldstein and Michael S Brown},
  journal={Cell},
  year={1985},
  volume={42},
  pages={203-212}
}
Cholesterol homeostasis is maintained by feedback inhibition of transcription of the gene encoding HMG CoA reductase. To study this mechanism, we joined the 5' end of the hamster reductase gene to the coding region for chloramphenicol acetyltransferase (CAT). The chimeric gene produced high levels of CAT activity in mouse L cells; sterols suppressed expression by 70% to 90%. Sequences responsible for both promotion and inhibition of transcription were distributed over 500 bp extending 300 bp… 
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A clone for a DNA binding protein was isolated that binds to the conserved SRE octanucleotide in both a sequence-specific and a single-strand--specific manner and is consistent with a role in sterol-mediated control of transcription.
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It is found that HMGR promoter constructs were sterol responsive in live animals, adding in vivo relevance to previous findings in cultured cells and concluding that in vivo electroporation is a convenient and powerful technique for the analysis of promoter elements in the livers of live animals.
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References

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TLDR
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TLDR
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TLDR
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