5′-Nucleotidase Plays a Key Role in Uric Acid Metabolism of Bombyx mori

  title={5′-Nucleotidase Plays a Key Role in Uric Acid Metabolism of Bombyx mori},
  author={Linmeng Tang and Dehong Yang and Yaohui Wang and Xu Yang and Kai Chen and Xingyu Luo and Jun Xu and Yujia Liu and Zhen Tang and Qianqian Zhang and Zhiwei Liu and Yongping Huang},
Uric acid (UA) is the end-product in the human purine metabolism pathway. The UA that accumulates in silkworm tissues is excreted as a nitrogen waste product. Here, we first validated that Bombyx mori has a homolog of the human gene that encodes the 5′-nucleotidase (5′N) involved in purine metabolism. The B. mori gene, Bm5′N, is located upstream of other genes involved in UA metabolism in the silkworm. Disruption of Bm5′N via the CRISPR/Cas9 system resulted in decreased UA levels in the… 

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