4D bioprinting: the next-generation technology for biofabrication enabled by stimuli-responsive materials

  title={4D bioprinting: the next-generation technology for biofabrication enabled by stimuli-responsive materials},
  author={Yi-Chen Li and Yu Shrike Zhang and Ali Akpek and Su Ryon Shin and Ali Khademhosseini},
Four-dimensional (4D) bioprinting, encompassing a wide range of disciplines including bioengineering, materials science, chemistry, and computer sciences, is emerging as the next-generation biofabrication technology. By utilizing stimuli-responsive materials and advanced three-dimensional (3D) bioprinting strategies, 4D bioprinting aims to create dynamic 3D patterned biological structures that can transform their shapes or behavior under various stimuli. In this review, we highlight the… 

Figures from this paper

3D Bioprinting: from Benches to Translational Applications.

The history of bioprinting and the most recent advances in instrumentation and methods are covered, and the requirements for bioinks and cells to achieve optimal fabrication of biomimetic constructs are focused on.

Bioresorbable Polymers: Advanced Materials and 4D Printing for Tissue Engineering

This review presents different bioresorbable advanced polymers and discusses the use in 4D printing for tissue engineering applications of Stimuli-responsive polymeric hydrogels, the most promising material for 4D bio-fabrication.

Four-Dimensional (Bio-)printing: A Review on Stimuli-Responsive Mechanisms and Their Biomedical Suitability

This work deploys the advantages and drawbacks of the mechanisms used to produce stimuli-responsive constructs, using a classification based on the target stimulus: humidity, temperature, electricity, magnetism, light, pH, among others.

Natural Origin Biomaterials for 4D Bioprinting Tissue‐Like Constructs

In this review, natural origin biomaterials and the stimuli that can be exploited for granting dynamic morphological features and functionalities post‐printing are highlighted and a broad overview of recent reports focusing on 4D‐bioprinted constructs for tissue engineering and regenerative medicine is provided.

Recent Advances in 4D Bioprinting

The recent advances of 4D bioprinting, including the mechanism, structure design principles, applications in biomedical engineering, and also the facing challenges are reviewed.

Polymeric Systems for Bioprinting.

A review of recent bioprinting innovations, such as increasing architectural complexity and cell viability in heterogeneous tissue constructs, which allow for the investigation of biological questions that could not be addressed before.

Smart and Biomimetic 3D and 4D Printed Composite Hydrogels: Opportunities for Different Biomedical Applications

This paper aims to review stimuli-responsive hydrogels according to the kinds of external changes and recent applications in biomedical and 4D bioprinting.

Toward next-generation bioinks: Tuning material properties pre- and post-printing to optimize cell viability

How cells should be considered during bioink synthesis, 3D printing, and post-printing processing is discussed and what has been reported thus far with regard to the relationships between bioink material properties and cells is discussed.



Biomatrices and biomaterials for future developments of bioprinting and biofabrication

A direct 3D cell printing system using inkjet and gelation techniques with inkjet droplets is developed, and it is found that it had good potential to construct 3D structures with multiple types of cells.

3D Bioprinting for Tissue and Organ Fabrication

3D bioprinting offers unprecedented versatility to co-deliver cells and biomaterials with precise control over their compositions, spatial distributions, and architectural accuracy, therefore achieving detailed or even personalized recapitulation of the fine shape, structure, and architecture of target tissues and organs.

3D bioprinting of tissues and organs

3D bioprinting is being applied to regenerative medicine to address the need for tissues and organs suitable for transplantation and developing high-throughput 3D-bioprinted tissue models for research, drug discovery and toxicology.

25th anniversary article: Engineering hydrogels for biofabrication.

This review focuses on the deposition process, the parameters and demands of hydrogels in biofabrication, with special attention to robotic dispensing as an approach that generates constructs of clinically relevant dimensions.

Advancing the field of 3D biomaterial printing

This perspective outlines considerations for addressing technical hurdles that, once overcome, will facilitate rapid advancement of 3D biomaterial printing as an indispensable tool for both investigating complex tissue and organ morphogenesis and for developing functional devices for a variety of diagnostic and regenerative medicine applications.

Bio-ink properties and printability for extrusion printing living cells.

Alginate (Alg) was selected as the major component of the 'bio-ink' formulations for extrusion printing of cells and the viability of primary myoblasts delivered as a myoblast/Alg-Gel bio-ink was not affected by the printing process, indicating that the Alg-gel matrix provides a potential means to print 3D constructs that may find application in myoregenerative applications.

Biomimetic 4D printing.

Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering. The natural analogues of such systems are

A 3D bioprinting system to produce human-scale tissue constructs with structural integrity

An integrated tissue–organ printer (ITOP) that can fabricate stable, human-scale tissue constructs of any shape is presented and the incorporation of microchannels into the tissue constructs facilitates diffusion of nutrients to printed cells, thereby overcoming the diffusion limit of 100–200 μm for cell survival in engineered tissues.