454 sequencing put to the test using the complex genome of barley

@article{Wicker2006454SP,
  title={454 sequencing put to the test using the complex genome of barley},
  author={Thomas M Wicker and Edith Schlagenhauf and Andreas Graner and Timothy J. Close and Beat Keller and Nils Stein},
  journal={BMC Genomics},
  year={2006},
  volume={7},
  pages={275 - 275}
}
During the past decade, Sanger sequencing has been used to completely sequence hundreds of microbial and a few higher eukaryote genomes. In recent years, a number of alternative technologies became available, among them adaptations of the pyrosequencing procedure (i.e. "454 sequencing"), promising a ~100-fold increase in throughput over Sanger technology… CONTINUE READING