4',6-Diamidino-2-phenylindole (DAPI) induces bundling of Escherichia coli FtsZ polymers inhibiting the GTPase activity.

Abstract

FtsZ (Filamentous temperature sensitivity Z) cell division protein from Escherichia coli binds the fluorescence probe DAPI. Bundling of FtsZ was facilitated in the presence of DAPI, and the polymers in solution remained polymerized longer time than the protofilaments formed in the absence of DAPI. DAPI decreased both the maximal velocity of the GTPase… (More)

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@article{Nova200746Diamidino2phenylindoleI, title={4',6-Diamidino-2-phenylindole (DAPI) induces bundling of Escherichia coli FtsZ polymers inhibiting the GTPase activity.}, author={Esteban Nova and Felipe Montecinos and Juan E Brunet and Rosalba Lagos and Octavio Monasterio}, journal={Archives of biochemistry and biophysics}, year={2007}, volume={465 2}, pages={315-9} }