4‐Quinolones cause a selective loss of mitochondrial DNA from mouse L1210 leukemia cells

@article{Lawrence19934QuinolonesCA,
  title={4‐Quinolones cause a selective loss of mitochondrial DNA from mouse L1210 leukemia cells},
  author={Jeffrey W. Lawrence and Sandra J. Darkin-Rattray and Feng Xie and Allen H. Neims and Thomas C. Rowe},
  journal={Journal of Cellular Biochemistry},
  year={1993},
  volume={51}
}
The 4‐quinolone antibiotics nalidixic acid and ciprofloxacin and potent inhibitors of the bacterial type II topoisomerase DNA gyrase. Treatment of mouse L1210 leukemia cells with these drugs resulted in a delayed inhibition of cell proliferation. Prior to inhibition of cell proliferation, there was a time‐dependent decrease in the cellular content of mitochondrial DNA (mtDNA). The decrease in mtDNA was associated with a decrease in the rate of mitochondrial respiration and an increase in the… 
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EFFECT OF CIPROFLOXACIN, VITAMIN E AND THEIR COMBINATION ON DNA FRAGMENTATION AND BONE MARROW CYTOGENICITY
The antibacterial activities of the fluorinated 4-quinolone, ciprofloxacin has been ascribed to a marked inhibition of bacterial DNA gyrase. However, evidence is accumulating that ciprofloxacin may
Calcium Signals Are Affected by Ciprofloxacin as a Consequence of Reduction of Mitochondrial DNA Content in Jurkat Cells
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Long-term exposure of Jurkat cells to ciprofloxacin at a concentration of 25 μg/ml seriously affects cellular energy metabolism and calcium homeostasis.
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References

SHOWING 1-10 OF 33 REFERENCES
Ciprofloxacin-induced inhibition of topoisomerase II in human lymphoblastoid cells.
TLDR
Using a nondenaturing filter elution method, a marked induction of double-strand DNA breaks in human lymphoblastoid cells exposed to 80 micrograms of ciprofloxacin per ml was seen, indicating that ciproprofloxin has an effect on intracellularly located topoisomerase II in humans.
Delayed cytotoxicity and selective loss of mitochondrial DNA in cells treated with the anti-human immunodeficiency virus compound 2',3'-dideoxycytidine.
TLDR
The mitochondrial toxicity and cell growth inhibition were reversed when ddC was removed and the reduction in cellular content of mitochondrial DNA caused by ddC may partially explain the delayed toxicity observed in acquired immunodeficiency syndrome patients treated with the drug.
Selective loss of mitochondrial DNA after treatment of cells with ditercalinium (NSC 335153), an antitumor bis-intercalating agent.
TLDR
Results suggest that ditercalinium exerts a specific cytotoxic effect at the level of mitochondrial DNA, which could account for the delayed cytotoxicity induced by this compound.
Effect of 4-quinolones and novobiocin on calf thymus DNA polymerase alpha primase complex, topoisomerases I and II, and growth of mammalian lymphoblasts.
TLDR
The antimicrobial agents did not significantly increase the frequencies of base pairing mismatches during the course of replication, indicating that the basal mutation rate is not affected by novobiocin and the 4-quinolones.
Effects of fleroxacin on HeLa cell functions and topoisomerase II.
TLDR
Judging from the serum levels attainable after oral administration, new 4-quinolones, especially fleroxacin, will have little effect on topoisomerase in eukaryotic cells.
Analysis of methylglyoxal bis(guanylhydrazone)-induced alterations of hamster tumor mitochondria by correlated studies of selective rhodamine binding, ultrastructural damage, DNA replication, and reversibility.
TLDR
The combined results demonstrate mitochondria to be a selective target of MGBG action, and define structu the concept of mitochondrial fusion in M GBG-treated cells.
The presence of two mitochondrial DNA topoisomerases in human acute leukemia cells.
TLDR
A study of DNA topoisomerases in mitochondria from human acute leukemia cells found two activities have been detected in these organelles, presumably a type II and a type I enzyme.
Selective alteration of mitochondrial function by Ditercalinium (NSC 335153), a DNA bisintercalating agent.
TLDR
The combined evidence indicates that the toxicity of Ditercalinium to murine leukemia cells (L1210) and Chinese Hamster lung cells (DC3F) is due to disruption of mitochondrial function.
The effect of bacterial DNA gyrase inhibitors on DNA synthesis in mammalian mitochondria.
TLDR
The overall depression of incorporation of labeled dATP into mtDNA, including the reduction of radioactivity incorporated into replicative intermediates, suggests a 'swivelase' role for the putative gyrase, and this hypothesis is further supported by results obtained on sucrose gradient centrifugation of heat-denatured, D-loop mtDNA.
Inhibitory effects of quinolone antibacterial agents on eucaryotic topoisomerases and related test systems
TLDR
The available data indicate that most quinolones examined are not highly inhibitory for eucaryotic topoisomerases or other enzymes involved in DNA replication, and corroborating positive in vivo test results have not been observed with these antimicrobial agents.
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