4,5-Diaminophthalhydrazide as a highly sensitive chemiluminescence derivatization reagent for α-dicarbonyl compounds in high-performance liquid chromatography

@inproceedings{Ishida199245DiaminophthalhydrazideAA,
  title={4,5-Diaminophthalhydrazide as a highly sensitive chemiluminescence derivatization reagent for α-dicarbonyl compounds in high-performance liquid chromatography},
  author={Junichi Ishida and Shinji Sonezaki and Masatoshi Yamaguchi},
  year={1992}
}
Abstract 4,5-Diaminophthalhydrazide (DPH) was found to be a highly sensitive chemiluminescence derivatization reagent for α-dicarbonyl compounds in high-performance liquid chromatography. The reagent reacts with α-dicarbonyl compounds in dilute hydrochloric acid in the presence of β-mercaptoethanol to give highly chemiluminescent quinoxaline derivatives which produce chemiluminescence by reaction with hydrogen peroxide and potassium hexacyanoferrate(III). The DPH derivatives of five… CONTINUE READING