4'-Amino-benzamido-taurocholic acid selectively solubilizes glycosyl-phosphatidylinositol-anchored membrane proteins and improves lipolytic cleavage of their membrane anchors by specific phospholipases.

@article{Mueller19944AminobenzamidotaurocholicAS,
  title={4'-Amino-benzamido-taurocholic acid selectively solubilizes glycosyl-phosphatidylinositol-anchored membrane proteins and improves lipolytic cleavage of their membrane anchors by specific phospholipases.},
  author={Guenter Mueller and A Kornd{\"o}rfer and K{\"u}lliki Saar and B Karbe-Th{\"o}nges and Hugo Fasold and Stefan M{\"u}llner},
  journal={Archives of biochemistry and biophysics},
  year={1994},
  volume={309 2},
  pages={329-40}
}
Glycosyl-phosphatidylinositol-anchored membrane proteins (GPI-proteins) are normally identified either by cleavage of the lipid anchor using (glycosyl)phosphatidylinositol-specific phospholipases C or D (GPI-PLs) or by metabolic labeling of the lipid moiety with specific building blocks. Therefore, methods for discrimination between transmembrane proteins and GPI-proteins on the basis of their physicochemical properties are desirable. Here we are presenting a selective extraction method for… CONTINUE READING