3D segmentation of keratin intermediate filaments in confocal laser scanning microscopy

  title={3D segmentation of keratin intermediate filaments in confocal laser scanning microscopy},
  author={Gerlind Herberich and Reinhard Windoffer and Rudolf E. Leube and Til Aach},
  journal={2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society},
In this paper, we propose and compare different methods for the 3D segmentation of keratin intermediate filaments (KFs) in images acquired using confocal laser scanning microscopy (CLSM). KFs are elastic cables forming a complex scaffolding within epithelial cells. They are involved in many basic cell functions. To understand the mechanisms of filament formation and network organisation under physiological and pathological conditions, quantitative measurements of dynamic network alterations are… CONTINUE READING
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