3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis.

@article{Harris20043DRO,
  title={3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis.},
  author={J. Robin Harris and Ulrich Meissner and Wolfgang Gebauer and J{\"u}rgen Markl},
  journal={Micron},
  year={2004},
  volume={35 1-2},
  pages={23-6}
}
Procedures are presented for the purification of the subunit dimer from Acanthochiton fasicularis hemocyanin. Electron microscopy of negatively stained specimens revealed a uniform population of macromolecules possessing the characteristic "boat shape". A 3D reconstruction from this EM data generated a approximately 3 nm resolution model that correlates well with earlier data of the purported subunit dimer, extracted from the 3D reconstruction of the didecamer of Haliotis tuberculata hemocyanin… CONTINUE READING