3D-printed microfluidic chips with patterned, cell-laden hydrogel constructs

  title={3D-printed microfluidic chips with patterned, cell-laden hydrogel constructs},
  author={Stephanie Knowlton and Chu Hsiang Yu and Fulya Ersoy and Sharareh Emadi and Ali Khademhosseini and Savas Tasoglu},
Three-dimensional (3D) printing offers potential to fabricate high-throughput and low-cost fabrication of microfluidic devices as a promising alternative to traditional techniques which enables efficient design iterations in the development stage. In this study, we demonstrate a single-step fabrication of a 3D transparent microfluidic chip using two alternative techniques: a stereolithography-based desktop 3D printer and a two-step fabrication using an industrial 3D printer based on polyjet… 

A 3D printed microfluidic perfusion device for multicellular spheroid cultures

A proof-of-concept in simplifying and integrating the prototyping and operation of a microfluidic spheroid culture device, which will facilitate its applications in various drug efficacy, metabolism and toxicity studies.

Accessing microfluidics through feature-based design software for 3D printing

The production of multiple microfluidic architectures using a hybrid 3D printing-soft lithography approach is demonstrated and shown to enable rapid device fabrication with channel dimensions that take advantage of laminar flow characteristics.

3D-Printed Microfluidics and Potential Biomedical Applications

3D printing is a smart additive manufacturing technique that allows the engineering of biomedical devices that are usually difficult to design using conventional methodologies such as machining or

Fabrication of truly 3D microfluidic channel using 3D-printed soluble mold.

The use of three-dimensional printed soluble wax as cast molds for rapid fabrication of truly arbitrary microfluidic polydimethylsiloxane (PDMS) channels that are not achieved through typical soft lithography is introduced.

3D printed microfluidic devices: a review focused on four fundamental manufacturing approaches and implications on the field of healthcare

In the last few years, 3D printing has emerged as a promising alternative for the fabrication of microfluidic devices, overcoming some of the limitations associated with conventional

3D printed microfluidic devices: a review focused on four fundamental manufacturing approaches and implications on the field of healthcare

In the last few years, 3D printing has emerged as a promising alternative for the fabrication of microfluidic devices, overcoming some of the limitations associated with conventional

The crossing and integration between microfluidic technology and 3D printing for organ-on-chips.

The combination of 3D printing and microfluidic technology in organ-on-chips provides a more efficient choice for building complex flow channels or chambers, as well as the ability to create biological structures with a 3D cell distribution, heterogeneity and tissue-specific function.

Projection-Based 3D Printing of Cell Patterning Scaffolds with Multiscale Channels.

A projection-based 3D printing system that achieves rapid and high-resolution fabrication of hydrogel scaffolds featuring intricate channels for multiscale cell patterning, and shows that red fluorescent protein-transfected A549 human nonsmall lung cancer cells adhered well in the scaffolds' channels, and showed further attachment and penetration during cell culture proliferation.

Can 3D Printing Bring Droplet Microfluidics to Every Lab?—A Systematic Review

It is concluded that today, 3D printers could already be used in every research lab and printing droplet microfluidics is also a possibility, albeit with some challenges discussed in this review.



Three-Dimensional Printing Based Hybrid Manufacturing of Microfluidic Devices.

A new way to fabricate stand-alone microfluidic devices with integrated manifolds and embedded microchannels by utilizing a 3D printing and laser micromachined lamination based hybrid manufacturing approach.

3D printing of soft lithography mold for rapid production of polydimethylsiloxane-based microfluidic devices for cell stimulation with concentration gradients

It is demonstrated that concentration gradients of growth factors, important regulators of cell/tissue functions in vivo, influence the survival and growth of human embryonic stem cells and could have strong implications for tissue engineering and drug screening.

Direct, one-step molding of 3D-printed structures for convenient fabrication of truly 3D PDMS microfluidic chips

Abstract In this work, we developed a convenient, one-step soft-lithographic-based molding technique for molding truly 3D microfluidic channels in polydimethylsiloxane (PDMS) by overcoming two grand

Maskless fabrication of cell-laden microfluidic chips with localized surface functionalization for the co-culture of cancer cells

A fabrication approach in which popular fabrication methods and techniques are coupled together to develop an integrated system that aids in the fabrication of cell-laden microfluidic systems and decreases the lengthy fabrication time is presented.

Research highlights: printing the future of microfabrication.

This issue highlights new work showing that 3D printing (stereolithography approaches in particular) has now risen as a viable technology to print whole microfluidic devices, and highlights recent work in which printing of membrane-bound droplets that interconnect through bilayer membranes may open up an entirely new approach to micro fluidic manufacturing of soft devices that mimic physiological systems.

3D-Printed Microfluidics.

3D printing will replace most PDMS and plastic molding techniques in academia in the next few years as structures are created by adding materials without the need for etching or dissolution, processing is environmentally friendly and economically efficient.

A 3D printed fluidic device that enables integrated features.

A reusable, high throughput, 3D printed fluidic device was created that enables flow and incorporates a membrane above a channel in order to study drug transport and affect cells and it is shown that mammalian cells cultured on this membrane can be affected by reagents flowing through the channels.

Cost-effective three-dimensional printing of visibly transparent microchips within minutes.

One-step fabrication of transparent three-dimensional (3D) microfluidic to millifluidic devices was demonstrated using a commercial 3D printer that employs dynamic mask projection stereolithography, allowing fast concept-to-chip time.