3D high- and super-resolution imaging using single-objective SPIM

@article{Galland20153DHA,
  title={3D high- and super-resolution imaging using single-objective SPIM},
  author={R{\'e}mi Galland and Gianluca Grenci and Ajay Aravind and Virgile Viasnoff and Vincent Studer and Jean-Baptiste Sibarita},
  journal={Nature Methods},
  year={2015},
  volume={12},
  pages={641-644}
}
  • Rémi Galland, Gianluca Grenci, +3 authors Jean-Baptiste Sibarita
  • Published in Nature Methods 2015
  • Biology, Medicine
  • Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam–steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations and features high background rejection and efficient photon collection, allowing for 3D single-molecule-based super-resolution imaging of whole cells or cell aggregates. Using larger… CONTINUE READING

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