3D Primary Culture Model to Study Human Mammary Development.

@article{Miller20173DPC,
  title={3D Primary Culture Model to Study Human Mammary Development.},
  author={Daniel H. Miller and Ethan S. Sokol and Piyush B. Gupta},
  journal={Methods in molecular biology},
  year={2017},
  volume={1612},
  pages={
          139-147
        }
}
We present a protocol for expanding human mammary tissues from primary patient-derived cells in three-dimensional (3D) cultures. The primary epithelial cells are seeded into 3D hydrogels with defined components, which include both proteins and carbohydrates present in mammary tissue. Over a span of 10-14 days, the seeded cells form mammary tissues with complex ductal-lobular topologies and include luminal and basal cells in the correct orientation, together with cells that stain positively for… CONTINUE READING
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