3D Image Super-Resolution by Fluorophore Fluctuations and MA-TIRF Microscopy Reconstruction (3D-COL0RME)

  title={3D Image Super-Resolution by Fluorophore Fluctuations and MA-TIRF Microscopy Reconstruction (3D-COL0RME)},
  author={Vasiliki Stergiopoulou and Luca Calatroni and S{\'e}bastien Schaub and Laure Blanc-F'eraud},
  journal={2022 IEEE 19th International Symposium on Biomedical Imaging (ISBI)},
We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution of a thin layer adjacent to the coverslip in Total Internal Reflection Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D - Covariance-based ℓ0 super-Resolution Microscopy with intensity Estimation) improves both lateral and axial resolution by combining sparsity-based modelling for precise molecule localisation and intensity estimation in the lateral plane with a 3D… 

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