3D DOSY-TROSY to determine the translational diffusion coefficient of large protein complexes.

  title={3D DOSY-TROSY to determine the translational diffusion coefficient of large protein complexes.},
  author={Tatiana Didenko and Rolf Boelens and Stefan G D R{\"u}diger},
  journal={Protein engineering, design & selection : PEDS},
  volume={24 1-2},
The translational diffusion coefficient is a sensitive parameter to probe conformational changes in proteins and protein-protein interactions. Pulsed-field gradient NMR spectroscopy allows one to measure the translational diffusion with high accuracy. Two-dimensional (2D) heteronuclear NMR spectroscopy combined with diffusion-ordered spectroscopy (DOSY) provides improved resolution and therefore selectivity when compared with a conventional 1D readout. Here, we show that a combination of… CONTINUE READING
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