3432 Microbiological study of daily dose eyedrops after 12 hours use


Aim: To evaluate the histological changes and immune response of conjuctiva in patients (pts) with lo S.S. Materials and Methods: We studied conjunctiial biopsies from 15 pts with I0 S.S. and 2 normal controls using 1) paraflfn section screening for the quality of conjunctival epithelium (CE), the number of goblet cells (GC) and the degree of inflammation, 2) Mo-abs and Pabs to: Tlymphocytes, T4, NK cells, &lymphocytes, K and A light chain and MHC antigens class II (HLA DR) and I (br microgl), 3) frown sections stained with Mo-ab to TS and direct I.F. for detection of Ig’s (IgA, IgM, IgM) and complement (C3, Clq, Cu) deposits. Results: The thickness of CE was focally or diffusely diminished in 9/15 pts. Desquampltton of the cells was found In 11/15 pts. Nine pts had a great reduction and 6 moderate reduction in number of GC. Two had normal numbers. Inflammation was severe in 2, moderate in 4, low in 2 and absent in 2 pts. A good correlation of findings between lip and conjuctiva biopses was observed in E/IO pts. In 13 pts with inflammation, T cells predominated, T4:TE ratio was about 2.3:1, NK cells were rare and plasma cells (found in 7/15 pts) were K+ > A+. In 4 cases uneven staining of CE for HLA-DR was found. Five cases showed mainly epithelial and 2 both CE and endothelial staining for brmicr. In 5/13 cases granular deposition of compl (C3 and/or C4) with Ig’s (IgM/IgG) in capillary wall was found. Controls were negative. Conclusion: In I0 S.S. 1) the predominant cell of the conjunctival inflammation is T-lymphocyte, 2) conjunctival epithelial cells may show increased expression of MHC class I and aberrant expression of class II and 3) capillary immune complex deposition can be also found.

DOI: 10.1016/0042-6989(95)90342-9

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@article{Dupin19953432MS, title={3432 Microbiological study of daily dose eyedrops after 12 hours use}, author={Olivier Dupin and C. Doumergue and S. Compeyre and Claude Trinquand and M T Zuccarelli and Bernard Arnaud}, journal={Vision Research}, year={1995}, volume={35} }