32P-adduct assay: Short- and long-term persistence of 2-acetylaminofluorene-DNA adducts and other applications of the assay

@article{Gupta198832PadductAS,
  title={32P-adduct assay: Short- and long-term persistence of 2-acetylaminofluorene-DNA adducts and other applications of the assay},
  author={Ramesh Chander Gupta},
  journal={Cell Biology and Toxicology},
  year={1988},
  volume={4},
  pages={467-474}
}
32P-Postlabeling techniques have been developed to detect and measure adducts formed by covalent binding of carcinogens of Known or unknown origin with DNA (and RNA). The assay is applicable to various classes of chemical carcinogens and permits detection of many adducts at attomole (10−18 mol) level using microgram amounts of DNA. Here, we demonstrate the application of the assay for the analysis of short- and long-term persistence of 2-acetylaminofluorene-DNA adducts in rat liver in vivo and… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 31 references

Analysis of DNA adducts by 32p-labeling and thin-layer chromatography

  • R. C. GUPTA, K. RANDERATH
  • DNA Repair,
  • 1988

Fresh cigarette

  • R. C. GUPTA, K. EARLEY, M. L. SOPOKI, C. G. GAIROLA
  • 1988

Postlabeling analysis of carcinogenDNA adducts in Mussel, Mytilus galloprovincialis

  • K. BRANKO, M. CHACKO, R. C. GUPTA
  • Marine Environ. Res
  • 1988

Tissue-specific DNA modifications in untreated, aged rats as detected by 32p-adduct assay

  • A. GARG, R. C. GUPTA
  • Proc. Am. Assoc. Cancer Res
  • 1988

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