31P-nuclear magnetic resonance analysis of the intracellular pH in the slime mold Dictyostelium discoideum.

@article{Satre198531PnuclearMR,
  title={31P-nuclear magnetic resonance analysis of the intracellular pH in the slime mold Dictyostelium discoideum.},
  author={Michel Satre and J B Martin},
  journal={Biochemical and biophysical research communications},
  year={1985},
  volume={132 1},
  pages={140-6}
}
Amoebae of the slime mold D. discoideum were studied by phosphorus nuclear magnetic resonance. Under aerobic conditions, major intracellular phosphate compounds included phosphomonoesters, inorganic phosphate (Pi), ADP and ATP. Nucleotides were essentially as magnesium complexes. Two intracellular Pi resonances were clearly resolved and the corresponding pHs determined by the chemical shifts characteristics were 7.7 and 6.7. These intracellular pHs were strictly constant over an extracellular… CONTINUE READING