31P NMR measurements of the ADP concentration in yeast cells genetically modified to express creatine kinase.

@article{Brindle199031PNM,
  title={31P NMR measurements of the ADP concentration in yeast cells genetically modified to express creatine kinase.},
  author={Kevin M Brindle and P. S. Braddock and Stephanie Fulton},
  journal={Biochemistry},
  year={1990},
  volume={29 13},
  pages={3295-302}
}
Rabbit muscle creatine kinase has been introduced into the yeast Saccharomyces cerevisiae by transforming cells with a multicopy plasmid containing the coding sequence for the enzyme under the control of the yeast phosphoglycerate kinase promoter. The transformed cells showed creatine kinase activities similar to those found in mammalian heart muscle. 31P NMR measurements of the near-equilibrium concentrations of phosphocreatine and cellular pH together with measurements of the total… CONTINUE READING