3 Screen ELISA for High-Throughput Detection of Beta Cell Autoantibodies in Capillary Blood.

@article{Ziegler20163SE,
  title={3 Screen ELISA for High-Throughput Detection of Beta Cell Autoantibodies in Capillary Blood.},
  author={Anette-G. Ziegler and Florian Haupt and Marlon Scholz and Katharina Weininger and Susanne Wittich and Stephanie L{\"o}bner and Claudia Matzke and Cigdem Gezginci and Stephanie Riethausen and Andreas Beyerlein and Stephanie Zillmer and Marie Amoroso and Rebecca L Coles and Michael Powell and Jadwiga Furmaniak and Bernard Rees Smith and C. Jurincic Winkler and Ezio Bonifacio and Peter Achenbach},
  journal={Diabetes technology & therapeutics},
  year={2016},
  volume={18 11},
  pages={
          687-693
        }
}
BACKGROUND Testing for beta cell autoantibodies is used for wide-scale identification of early stages of type 1 diabetes. This requires suitable screening assays. We aimed to establish screening that utilized a first step assay (3 Screen) able to detect autoantibodies to the target antigens glutamic acid decarboxylase-65 (GAD), insulinoma-associated antigen 2 (IA-2), and zinc transporter 8 (ZnT8) to identify children positive for multiple beta cell autoantibodies. METHODS An ELISA format was… CONTINUE READING