3-Nitrotyrosine as a spectroscopic probe for investigating protein protein interactions.

  title={3-Nitrotyrosine as a spectroscopic probe for investigating protein protein interactions.},
  author={Vincenzo De Filippis and R. P. M. Frasson and Angelo Fontana},
  journal={Protein science : a publication of the Protein Society},
  volume={15 5},
3-Nitrotyrosine (NT) is approximately 10(3)-fold more acidic than Tyr, and its absorption properties are strongly pH-dependent. NT absorbs radiation in the wavelength range where Tyr and Trp emit fluorescence (300-450 nm), and it is essentially nonfluorescent. Therefore, NT may function as an energy acceptor in resonance energy transfer (FRET) studies for investigating ligand protein interactions. Here, the potentialities of NT were tested on the hirudin thrombin system, a well-characterized… CONTINUE READING


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